Bone Health - Bone & Mineral Health Screening Panel
- Free home sample collection
- Accurate & trusted reports
- Pathologist-verified reports
Vitamin D deficiency screening - bone health, immunity and fatigue.
Also known as: 25-Hydroxy Vitamin D, Vitamin D Total, Calcidiol
Sample
Blood
Fasting
Not required
Report in
24 hours
Collection
Free at home
The Vitamin D (25-OH) test measures the main circulating form of vitamin D in your blood. Vitamin D is essential for calcium absorption, bone health, and immune function, and deficiency is extremely common - especially in people with limited sun exposure, darker skin, or a vegetarian diet.
Ordered to investigate bone or muscle pain, fatigue, frequent illness, or as part of routine wellness screening, since deficiency is common and often silent. Also checked in people with osteoporosis, malabsorption conditions, limited sun exposure, or during pregnancy.
It can help your doctor assess
Vitamin D deficiency and insufficiency, which are linked to weakened bones (osteomalacia/osteoporosis risk), muscle weakness, and (in children) rickets.
This test may be considered for:
Whether this test is right for you depends on your symptoms, medical history and the reason for testing - your doctor can advise.
25-hydroxyvitamin D (the combined D2 + D3 form), which is the most accurate indicator of overall vitamin D status in the body.
25-OH Vitamin D (Total)
Vitamin D Status
Combines both D2 (from plant/supplement sources) and D3 (from sunlight/animal sources) to give the best overall picture of your vitamin D reserves.
How to read your result
Your report should be read using the reference interval, unit and method shown by the performing laboratory. A result outside a reference interval does not automatically establish a diagnosis, and a result inside the interval does not rule out every health problem. Clinical interpretation depends on symptoms, history and other findings.
| Marker | Result | What it may be associated with |
|---|---|---|
| 25-OH Vitamin D | Low | Vitamin D deficiency - associated with bone pain, muscle weakness, and increased fracture risk over time. Very common and usually corrected with supplementation and sun exposure. |
| 25-OH Vitamin D | Very High | Rare, usually from excessive supplementation - can raise blood calcium to unsafe levels and needs medical attention. |
If a result is outside the range
If the Vitamin D (25-OH) is reported as high, low, positive, negative or otherwise outside the laboratory's stated interval, the meaning depends on the specific test and the reason it was ordered. Your clinician may consider repeat testing, related investigations or other clinical information before deciding what the result means.
Limitations of this test
The test shows overall vitamin D status but not the cause of deficiency (diet vs. sun exposure vs. absorption issue) - your doctor will use your history to guide that.
An abnormal result does not by itself mean you have a particular disease. Your doctor interprets results together with your symptoms, history and other tests.
| Parameter | Reference range | Unit |
|---|---|---|
| 25-OH Vitamin D | 30 - 100 (sufficient) | ng/mL |
| 25-OH Vitamin D | 20 - 29 (insufficient) | ng/mL |
| 25-OH Vitamin D | Below 20 (deficient) | ng/mL |
Typical adult ranges for guidance. Reference intervals vary with the laboratory method, age and sex - always read your result against the range printed on your own report.
Book
Choose the test and a time slot online, or call us and we'll book it for you.
Prepare
Follow the preparation steps above for this test.
Sample collection
A trained phlebotomist collects the sample at your home - no extra charge.
Laboratory processing
Your sample is processed in our laboratory and reviewed by a pathologist.
Report
Your report is shared on WhatsApp and email, usually within 24 hours.
For a blood-based test, a small blood sample is collected by a trained healthcare professional, usually from a vein in the arm. The collection normally takes only a few minutes; mild temporary discomfort or bruising can occur at the puncture site.
Season (levels are typically lower after winter/monsoon with less sun exposure), skin tone, sunscreen use, body weight, and recent supplementation can all influence the result.
Laboratory results are interpreted together with your symptoms, medical history and other investigations. If your results are significantly abnormal, or you have symptoms that worry you, discuss them with a qualified doctor promptly.
Trusted public health sources for the information on this page:
This information is for general awareness and does not replace advice from your doctor.
The Vitamin D (25-OH) test cost in Mumbai at HWP Diagnostic is ₹799 - 72% less than the MRP of ₹2,899. There are no extra charges for home sample collection. The same price applies across Malad, Kandivali, Borivali and Goregaon.
| Test | Vitamin D (25-OH) |
|---|---|
| Price at HWP Diagnostic | ₹799 |
| MRP | ₹2,899 (you save ₹2,100 - 72% off) |
| Home collection charge | Free |
| Report time | 24 hours |
| Fasting | Not required |
| Available in | Malad, Kandivali, Borivali & Goregaon (same price) |
Book the Vitamin D (25-OH) test with free home sample collection anywhere in Malad, Kandivali, Borivali and Goregaon, or walk in to our lab in Malad West, Mumbai - open 24×7.
Home collection areas include
Malad WestMalwani & KharodiMalad EastKandivali WestKandivali EastBorivali WestGorai & Borivali WestIC Colony & MandapeshwarBorivali EastGoregaon WestBangur Nagar & Motilal NagarGoregaon EastAarey ColonyDahisarJogeshwari WestJogeshwari East
Directions on Google MapsIt is also known as 25-Hydroxy Vitamin D, Vitamin D Total, Calcidiol.
Home collection at the same price (₹799) across these areas, with our lab in Malad West open 24×7.
Get this test along with other important checks - usually for less than booking them separately.
No fasting is required for this test. You can have it done at any time.
Despite abundant sunlight, indoor lifestyles, pollution, extensive clothing coverage, sunscreen use, and darker skin (which needs more sun exposure to produce vitamin D) all contribute to widespread deficiency.
Typically with vitamin D supplements (dose depends on severity) alongside safe sun exposure and dietary sources like fortified foods and fatty fish. Your doctor will guide dosage and follow-up testing.
Usually after 8-12 weeks of supplementation, to check whether levels have improved and adjust the dose if needed.
Yes, excessive supplementation (well beyond recommended doses) can raise blood calcium to unsafe levels - always follow your doctor’s prescribed dosage rather than self-medicating with high-dose supplements.
It’s one of several contributing factors that can be associated with hair thinning, alongside iron, thyroid and other nutritional factors - your doctor may check multiple markers together.
Vitamin D (25-OH) is a blood test. Vitamin D deficiency screening - bone health, immunity and fatigue.
The Vitamin D (25-OH) may be ordered when a healthcare professional needs information about the condition or health area assessed by this test. Vitamin D supports bones, immunity and mood, yet deficiency is widespread even in sunny climates. This test tells you whether you need supplementation and by how much.
Vitamin D deficiency screening - bone health, immunity and fatigue.
The test may be relevant for people whose symptoms, medical history, screening needs or treatment monitoring match the purpose of the Vitamin D (25-OH).
No fasting required.
No, fasting is not required for the Vitamin D (25-OH). You can eat and drink normally unless your doctor has advised otherwise.
The Vitamin D (25-OH) is done on a blood sample.
For a blood-based test, a small blood sample is collected by a trained healthcare professional, usually from a vein in the arm. The collection normally takes only a few minutes; mild temporary discomfort or bruising can occur at the puncture site.
Reports for the Vitamin D (25-OH) are usually ready within 24 hours of sample collection. You receive them on WhatsApp and email.
The Vitamin D (25-OH) costs Rs 899 at HWP Diagnostic (MRP Rs 1800). Home sample collection is free.
Your report should be read using the reference interval, unit and method shown by the performing laboratory. A result outside a reference interval does not automatically establish a diagnosis, and a result inside the interval does not rule out every health problem. Clinical interpretation depends on symptoms, history and other findings.
If the Vitamin D (25-OH) is reported as high, low, positive, negative or otherwise outside the laboratory's stated interval, the meaning depends on the specific test and the reason it was ordered. Your clinician may consider repeat testing, related investigations or other clinical information before deciding what the result means.
The Vitamin D (25-OH) can provide important information, but laboratory results are generally interpreted together with symptoms, medical history, examination and other investigations. A single result should not be treated as a diagnosis without clinical context.
Some medicines, supplements, food, exercise and other pre-test factors can affect particular laboratory results. Tell the healthcare professional about medicines and supplements you take, and do not stop prescribed medicines unless your clinician tells you to.